New Delhi Metallo-Beta-Lactamase–Producing Enterobacteriaceae
New Delhi Metallo-Beta-Lactamase–Producing Enterobacteriaceae
Pulsed-field gel electrophoresis (PFGE) was performed by using the CHEF mapper electrophoresis system (Bio-Rad Laboratories) with XbaI-digested K. pneumoniae and E. coli chromosomal DNA, as described for E. coli (www.cdc.gov/pulsenet/protocols.htm). PFGE patterns were compared by using the Dice coefficient and clustering by using the unweighted-pair group method with average linkages (Bionumerics version 5.10; Applied Maths Inc., Austin, TX, USA).
Pulsed-field Gel Electrophoresis
Pulsed-field gel electrophoresis (PFGE) was performed by using the CHEF mapper electrophoresis system (Bio-Rad Laboratories) with XbaI-digested K. pneumoniae and E. coli chromosomal DNA, as described for E. coli (www.cdc.gov/pulsenet/protocols.htm). PFGE patterns were compared by using the Dice coefficient and clustering by using the unweighted-pair group method with average linkages (Bionumerics version 5.10; Applied Maths Inc., Austin, TX, USA).